In typing by PCR amplification with sequence-specific primers (PCR-SSP), typing specificity is part of the amplification step, which makes the technique almost as fast as serological tissue typing. In the present study primers were designed for DR "low-resolution" typing by PCR-SSP, i.e. identifying polymorphism corresponding to the serologically defined series DR1-DRw18.

The B-L beta II family loci were amplified from genomic DNA by B-L beta II family specific primers and then characterized by PCR-SSP. In total, ten pairs of primers, derived from the sequences of expressed B-L beta II family alleles, were used in the PCR typing test to discriminate the chicken B haplotypes identified previously by serological means.

Sequence-specific Primer (PCR-SSP) Technology Analysis of the human leukocyte antigen (HLA) type is an important component of immunological research and biopharmaceutical research. Traditionally, micronucleotoxicity (MLCT) and flow cytometry (FC) were widely used to detect HLA types. PCR-SSP TYPING. M. Bunce; M. Bunce. Transplantation Immunology, Nuffield Department of Surgery, Oxford Transplant Centre, Churchill Hospital, Oxford, OX3 7LJ, England Molecular PCR typing allowed for precise antigen determination in all the patients. Comparing both methods we found PCR-SSP HLA typing method very useful in routine HLA-DR determination, especially valuable in patients, in whom some problems in serological testing are expected.

Pcr-ssp typing

PCR-SSP TYPING. M. Bunce; M. Bunce. Transplantation Immunology, Nuffield Department of Surgery, Oxford Transplant Centre, Churchill Hospital, Oxford, OX3 7LJ, England In forensics, the specific ABO blood group is often determined by analyzing the ABO gene. Among various methods used, PCR employing sequence-specific primers (PCR-SSP) is simpler than other methods for ABO typing.

General information on PCR-SSP using Phototyping methods.

PCR-SSP TYPING. M. Bunce; M. Bunce. Transplantation Immunology, Nuffield Department of Surgery, Oxford Transplant Centre, Churchill Hospital, Oxford, OX3 7LJ, England

In total, ten pairs of primers, derived from the sequences of expressed B-L beta II family alleles, were used in the PCR typing test to discriminate the chicken B haplotypes identified previously by serological means. Typing results of PCR-SSP, different from that of serology, were all confirmed by sequencing-based typing of HLA-DRB1 alleles.

Current DNA-based methods that are in use for HLA typing are polymerase chain reaction-sequence-specific priming (PCR-SSP). Polymerase chain reaction-

Application of a simple in-house PCR-SSP technique for HLA-B* 27 typing in spondyloarthritis patients. Discrepant HLA typing results were detected in 6 of 33 samples (18.2%) analyzed by two different PCR-SSO techniques and one PCR-SSP 1999-08-15 · We and others have shown that the sensitivity of polymerase chain reaction with sequence-specific primers (PCR-SSP) typing for HLA class II alleles can be increased 100-fold by the addition of a primary amplification step (nested PCR-SSP).

Integrated detection of the nullalleles A*24:09N, B*51:11N, C*04:09N, DRB4*01:03:01:02N, DRB5*01:08N. Integreated negative control. Short analysis due to allele specific amplification.
Vad är feminin

2018-10-05 Nested PCR-SSP is a two-step approach to HLA typing whereby the region of interest is amplified in the first step and this amplicon is used instead of genomic DNA for the second, sequence-specific amplifications, using primers which are internal to the first pair of amplification primers. Molecular genetic blood group typing by the use of PCR‐SSP technique Molecular genetic blood group typing by the use of PCR‐SSP technique Prager, Martina 2007-07-01 00:00:00 BACKGROUND: DNA‐based methods are useful for enhancing immunohematology typings. Ready‐to‐use Conformité Européenne (CE)‐marked test kits based on polymerase chain reaction with sequence‐specific priming We blindly typed these samples by in-house PCR-SSP technique and checked with HLA alleles of the same samples.

Dispensing primer mixes. Setting up PCR-SSP using TDMH buffer. Setting up PCR-SSP using heparin-contaminated DNA. PCR amplification program.
Medicinsk svenska ljud

In conclusion, we have successfully developed a simple, convenient, and cost-effective PCR-SSP technique for HLA-B * 27 typing which is a reliable diagnostic test for AS and related SpA diagnosis. This test can now be routinely applied for HLA-B * 27 genotyping in all tissue typing laboratories.Figure 1 :1Gel photograph showing B * 27 specific band with the control bands.

The mixtures were then subjected to nested PCR-SSP typing and analyzed by agarose gel electrophoresis. Typing results of PCR‐SSP, different from that of serology, were all confirmed by sequencing‐based typing of HLA‐DRB1 alleles.

Eur 14

Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail.

CONCLUSION: PCR-SSP is a helpful supplementary technique for resolving most of the common problems caused by discrepant or doubtful serologic results, and it is an easy-to-handle robust method. Questionable cases in donor, recipient, and patient typing can be examined with acceptable cost. T he molecular genetic basis of almost all blood INTRODUCTION Fast HLA typing based on sequence-specific primers is ideally suited for smaller numbers of samples and confirmatory tests.

In typing by PCR amplification with sequence-specific primers (PCR-SSP), typing specificity is part of the amplification step, which makes the technique almost as fast as serological tissue typing.

Gel photography. Interpretation of results. Notes . Testing control solutions. Testing allele-specific primers. Testing TDMH. Troubleshooting This chapter describes a polymerase chain reaction sequence-specific primer (PCR-SSP) typing system that is applicable to the identification of all HLA class I and class II alleles, as well as alleles from non-HLA loci (10, 11) .

Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more HLA Kits.